Pglo

2/15/2013 oscilloscope on transformation of bacteria with pGLO plasmid Experiment 5 pop the question Purpose of this lab is to have plasmid activity transformed solid Bacteria starter plate, pGLO DNA Plasmid, microcentrifuge pipages, Ice, water bath, CaCl2 Transformation response, (LB) agar plate, (LB/ angstrom unit) agar plate, (LB/Amp/ara) agar plate, Micropipette, and Micropipette tips. Method Genetic transformation is a mathematical function which is done by taking divisors from one organism and putting them in other organism.A gene is a piece of DNA that instruct for making a new protein and from this protein organism a certain trait. A gene is inserted into an organism in order to change the organisms trait. This unconscious process lab is divided into two day lab. On day one, we started the subprogram with getting agar plate where HB101 bacteria were growing for 24 hours at 37C. We began by first labeling two micro provides one with (+pGLO) and second with (-pGLO). 250ul of transformation solution which we used (CaCl2) was transfer to each tubes and set those tubes on field glass.HB101 bacteria superstar colony was picked by using sterile inoculation loop and immersed into (+pGLO) tube and later immersed into (-pGLO) using same technique. Both time we used antithetical sterile inoculation loop. The tubes were placed back into the ice after premix well the colony each time. The pGLO plasmid DNA was added by the instructor into (+pGLO) not into (-PGLO) tube and placed the tube back into ice. The tubes were incubated on ice for 10 minutes. erst done incubating both tubes were performed heat shocks at 42 degree C temperature for 50 second.Both tubes were immediately placed into the ice for another 2 minutes. After 2 minutes, 250ul of LB broth was added to each tube and again incubated for 10 minutes at room temperature. Once the incubation was done, we transferred 100ul of cell suspension to the plates which was provided by using the table LB/Amp LB/Amp/ara LB/Amp LB (+pGLO) (+pGLO) (-pGLO) (-pGLO) Once the cell suspension was transferred, cells were gently spread 10 swipes using inoculation loop on the agar and rotated the plate 45 degree. The plates were placed into incubator at 37 degrees by turning he tubes peak down and taping them. Result